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. 1997 Jan 20;185(2):305–316. doi: 10.1084/jem.185.2.305

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Induction of MHC class I heavy chain, β2-microglobulin and TAP1/TAP2 gene transcripts in individual neurons by IFN-γ. (A) Neurons pretreated with IFN-γ (100 U/ml) for 72 h. Gene transcripts for MHC class I heavy chain, β2-microglobulin (β2-m) and GAPDH coamplified in multiplex from individual neurons (lanes 1–10). Gene transcripts for TAP1 and TAP2 coamplified in parallel from the same neuron derived samples. Lanes N and M show negative control of PCR-amplification and molecular weight marker ΦX174/Hae III, respectively. Neurons pretreated with IFN-γ plus TTX for 72 h. Lane arrangement like in A.