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. 1997 Feb 17;185(4):601–608. doi: 10.1084/jem.185.4.601

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Purification of p17 subunits and direct inhibition of reconstituted CPP-32- and ICEsubunits by NO. (A) Silver stain of the crude homogenates before purification (60 μg; lane 3), the unbound fraction of the NiNTA columns (60 μg; lane 4) and the purified CPP-32 p17 subunit (10 μg; lane 2) separated by a 12% SDS–polyacrylamide gel. (B) Inhibition of cloned, purified and reconstituted CPP-32 and ICE by incubation with SNP or SNAP for 1 h.

Purification of p17 subunits and direct inhibition of reconstituted CPP-32- and ICEsubunits by NO. (A) Silver stain of the crude homogenates before purification (60 μg; lane 3), the unbound fraction of the NiNTA columns (60 μg; lane 4) and the purified CPP-32 p17 subunit (10 μg; lane 2) separated by a 12% SDS–polyacrylamide gel. (B) Inhibition of cloned, purified and reconstituted CPP-32 and ICE by incubation with SNP or SNAP for 1 h.