Table 2.
Grouping of Patient Isolate env Clones within pCR3 Based upon Restriction Digests and Choice of Predominant Clones for Preparation of vv
| Patient | Age | Group | Env clones* | Recombinant vv | ||||
|---|---|---|---|---|---|---|---|---|
| VI-05 | 2 mo | 05-2M-A | 3-2, 3-4, 3-5, 3-7, 3-8, 3-10 | vvVI-05-2M | ||||
| 05-2M-B | 3-3 | |||||||
| 6 mo | 05-6M-A | 4-2, 4-4, 4-5, 4-6, 4-8, 4-9 | vvVI-05-6M | |||||
| VI-06 | Birth (1 d) | 06-1D-A | 1-2, 1-3 | |||||
| 06-1D-B | 1-9 | |||||||
| 06-1D-C | 1-11, 1-12, 1-13, 1-15, 1-20 | vvVI-06-ID | ||||||
| 06-1D-D | 1-18 | |||||||
| 6 mo | 06-6M-A | 2-1, 2-2, 2-3, 2-4, 2-7, 2-8, 2-9, 2-10 | ‡ | |||||
| 06-6M-B | 2-5 | ‡ | ||||||
| VI-08 | 20 d | 08-20D-A | 5-4 | |||||
| 08-20D-B | 5-6 | |||||||
| 08-20D-C | 5-2, 5-3, 5-8, 5-9, 5-10 | vvVI-08-20D | ||||||
| 6 mo | 08-6M-A | 6-1 | ||||||
| 08-6M-B | 6-2, 6-3, 6-4, 6-7, 6-8, 6-9, 6-10 | vvVI-08-6M | ||||||
| VI-11 | 1 mo | 11-1M-A | 7-1 | |||||
| 11-1M-B | 7-2, 7-5, 7-7, 7-10, 7-11, 7-16, 7-19 | vvVI-11-1M | ||||||
| 11-1M-C | 7-12 | |||||||
| 3 mo | 11-3M-A | 8-3 | ||||||
| 11-3M-B | 8-4, 8-11, 8-14, 8-16 | vvVI-11-3M |
*
env genes amplified by PCR and ligated into pCR3 (InVitrogen); clones chosen for preparation of vv are in bold type.
‡
vv not made from this time point due to instability of the env insert.