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. 2001 Oct 15;155(2):279–290. doi: 10.1083/jcb.200102106

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Copyright © 2001, The Rockefeller University Press

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Figure 5. — Fusion pore dynamics revealed by continuous photobleaching. (A) Exemplified I_{FM 1-43} recordings obtained from released LBs, LBs within fused vesicles, and LBs within transiently (as in Fig. 2 D) fused vesicles. Steady-state I_{FM 1-43} reflects a balanced rate between dye bleaching and replenishment. (B) Confocal z sections of a single fused LB at times indicated in the upper diagram during a continuous photobleaching experiment (identical LSM gain setting in 1–3). Despite the apparent disappearance of fluorescence during steady state, the LB was still visible by contrast enhancement (I_{FM 1-43} >0). The shift in steady-state I_{FM 1-43} reflects spontaneous fusion pore expansion. (Bottom right) I_{FM 1-43} tracings of two LBs from the same cell, demonstrating the differential effect of ionomycin on the steady-state I_{FM 1-43}.