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. 1997 Oct 20;186(8):1213–1221. doi: 10.1084/jem.186.8.1213

Table 3.

Cytokine Release From CTL Generated In Vivo by Immunization with Bone Marrow–derived DCs

Splenocytes from mice immunized with: Stimulators
mIFN-γ (pg/ml/24 h)
CT26/P1A* CT26/βgP‡ CT26.CL25
HBSS   0        0      0
DC–β-gal transduced 266 216,700 151,100
DC–Neu transduced 164      210    372
Splen–βgP pulsed   0        0      0
DC–βgP pulsed   5     1197   1436
DC–P1A pulsed 420     351    477
*

CT26 tumor cells pulsed with P1A peptide (10 μg/ml).  

‡

CT26 tumor cells pulsed with βgP (β-gal Ld peptide) (10 μg/ml). Splenocytes from animals immunized with transduced or peptide-pulsed DCs or peptide-pulsed splenocytes were pooled and restimulated in vitro with the Ld-restricted β-gal peptide (βgP) for 6 d in EHAA media with 30 IU/ml IL-2 (as described in Materials and Methods). On day 7, restimulated splenocytes (1 × 105) were plated in flat-bottomed 96-well tissue culture plates with 105 peptide-pulsed tumor targets. After 24 h, the supernatant was collected and analyzed for IFN-γ content.