Figure 2.
The binding of importin β to nucleoporins in the solid phase assay is sensitive to RanGTP. The binding of 10 nM importin β to nucleoporins adsorbed to microtiter wells was analyzed in the absence of RanGTP, or in the presence of 20 or 100 nM RanGTP, as indicated. The bound importin β was quantified as described in the legend to Fig. 1.