Figure 1.
BCR crosslinking results in hyperphosphorylation of Akt/PKB in DT40 cells. (A) DT40 cells were stimulated with 10 μg/ml mouse anti-chicken IgM (M4) mAb for the indicated times. Equal amounts of cell lysates were immunoprecipitated with anti-Akt Ab (Santa Cruz Biotechnology) and immunoblotted with anti-Akt (Lower) or anti-phosphoSer-473-Akt specific Ab (Upper). (B) DT40 cells were stimulated with 10 μg/ml M4 after preincubation in the presence or absence of okadaic acid. The phosphorylation state of Akt was measured as described in A.