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Karyotyping markers included in the eight-probe mix
| DNA sequences | Fluorescent molecules | Individual cosmid use final concentration (ng/μl) | 19-cosmid mix use final concentration (ng/μl) |
|---|---|---|---|
| Knob 180 bp | Coumarin or Cascade Blue | 12.5 | 15.1 |
| 4-12-1 | FITC or Alexa Fluor 488 | 12.5 | 15.1 |
| NOR-173 | FITC or Alexa Fluor 488 | 0.0625 | 0.08 |
| Cent C | FITC or Alexa Fluor 488 | 0.5 | 0.6 |
| 2-3-3 | FITC or Alexa Fluor 488 | 3.125 | 3.8 |
| Cent 4 | Cy5 | 12.5 | 15.1 |
| 1-26-2 | Cy5 | 6.25 | 7.5 |
| 2-3-3 | Cy5 | 6.25 | 7.5 |
