Table 1.
Basal Eosinophil Counts in the p50+/+ and p50−/− Mice
| Bone marrow | Peripheral blood | |||
|---|---|---|---|---|
| % | % | |||
| p50+/+ mice | 7.2 ± 0 | 2.25 ± 0.75 | ||
| p50−/− mice | 5.0 ± 0.8 | 3.35 ± 0.35 |
Bone marrow cells were obtained by flushing the cavity of a single femur from each mouse with serum-free RPMI 1640 medium containing heparin (10 U/ml). Cells derived from the bone marrow and peripheral blood were suspended in lysis buffer (Pharm Lyse; PharMingen) for 2 min for lysis of the red blood cells. The cells were washed twice in PBS and cytospin preparations were stained with Diff-Quik. Shown are the number of eosinophils determined as a percentage of the total number of leukocytes ± SEM.