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. 2005 Apr 4;201(7):1061–1067. doi: 10.1084/jem.20042276

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Figure 1. — Decrease in peripheral CD4⁺CD25⁺ T cells in Tgf-β1 ^−/− mice. Thymocytes (a) and splenocytes (b and c) from 8–10-d-old Tgf-β1 ⁻ ^/ ⁻ mice or littermate controls were counted and stained for CD4 and CD8 and analyzed by flow cytometry. Peripheral CD4 T cells from Tgf-β1 ^−/− mice (gray line) or littermate controls (black line) were stained for CD44 and CD62L. Thymocytes and splenocytes from 8–10-d-old Tgf-β1 ⁻ ^/ ⁻ mice or littermate controls were stained for CD4 and CD25 and analyzed by flow cytometry (d). Proportion of CD4⁺CD25⁺ cells among CD4 T cells (e, n = 20).