Figure 7.
NK helper cells promote the DC-induced development of Th1 cells. In vitro Th cell priming, staphylococcal enterotoxin B–coated LPS-matured DCs were used to stimulate naive CD4+ Th cell proliferation in the absence or presence of γ-irradiated (2,000 rad) IL-18–induced NK helper (NKh) cells. At day 10, the resulting Th cell cultures were restimulated in neutral conditions with activating anti-CD3 and anti-CD28 antibodies. 24-h supernatants were tested for the presence of the respective Th1- and Th2-type cytokines, IFN-γ and IL-5. Similar data were obtained in one additional experiment.