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. 2005 Oct 3;202(7):901–906. doi: 10.1084/jem.20050784

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — Phenotypic analysis of neonatal and adult CD4 SP thymocytes. (A) Representative flow cytometric analysis of CD25 and Foxp3^gfp expression on CD4 SP thymocytes from a 6-wk-old mouse. The numbers indicate the percentage of cells in each gate. (B) Representative flow cytometric analysis of CD25 and Foxp3^gfp expression on CD4 SP thymocytes from a 1-d-old mouse. The numbers indicate the percentage of cells in each gate. (C) Comparison of the indicated cell surface marker expression on postnatal day 1 CD25⁺Foxp3^gfp− CD4 SPs with adult Foxp3/CD25-expressing subpopulations. Closed colored histograms are shown for cells from each of the adult Foxp3 × CD25 subpopulations and correspond to the gated population of the same color and position in the plot in A (blue, CD25^lo/negFoxp3^gfp+; green, CD25^hiFoxp3^gfp+; red, CD25⁺Foxp3^gfp−; gray, CD25⁻Foxp3^gfp−). In every case, the open histogram overlay (black) shows the corresponding marker expression by the postnatal day 1 CD25⁺Foxp3^gfp− CD4 SP population as defined by the bottom right gate in B.