Table II.
Recovery from Inactivation in Two-electrode Voltage-clamp Experiments
| τ−60mV (ms) | n | τ−90mV (ms) | n | τ−120mV (ms) | n | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| F1483Q | 18.28 | 1 | 2.98 ± 0.07 | 3 | ||||||||
| D1487A | 14.63 ± 0.59 | 3 | 2.26 ± 0.28 | 3 | 0.80 ± 0.08 | 3 | ||||||
| D1487N | 15.14 ± 0.17 | 3 | 2.95 ± 0.16 | 3 | 0.78 ± 0.05 | 3 | ||||||
| F1489A | 2.47 ± 0.36* | 3 | 0.87 ± 0.10* | 3 | 0.37 ± 0.04* | 4 | ||||||
| F1489C | 4.30 ± 0.11* | 3 | 0.89 ± 0.01* | 3 | 0.43 ± 0.01* | 3 | ||||||
| F1489H | 2.70 ± 0.09* | 4 | 0.74 ± 0.02* | 4 | 0.35 ± 0.02* | 4 | ||||||
| F1489I | 4.32 ± 0.29* | 4 | 1.00 ± 0.06* | 4 | 0.39 ± 0.04* | 4 | ||||||
| F1489L | 14.28 ± 1.06 | 4 | 1.88 ± 0.28 | 4 | 0.64 ± 0.06 | 4 | ||||||
| F1489M | 8.38 ± 0.31* | 3 | 1.49 ± 0.10* | 3 | 0.56 ± 0.04* | 3 | ||||||
| F1489Q | 1.19 ± 0.06* | 3 | 0.57 ± 0.02* | 3 | ||||||||
| F1489S | 1.91 ± 0.13* | 3 | 0.69 ± 0.07* | 3 | ||||||||
| F1489T | 2.83 ± 0.12* | 3 | 0.90 ± 0.04* | 3 | 0.39 ± 0.02* | 3 | ||||||
| F1489V | 2.41 ± 0.16* | 2 | 0.76 ± 0.03* | 4 | 0.35 ± 0.03* | 2 | ||||||
| F1489W | 13.33 ± 0.35* | 4 | 2.06 ± 0.18 | 4 | 0.65 ± 0.09 | 4 | ||||||
| F1489Y | 9.89 ± 0.76* | 4 | 1.69 ± 0.10* | 4 | 0.58 ± 0.04* | 4 | ||||||
| I1488F/F1489Q | 1.17 ± 0.12* | 3 | 0.46 ± 0.04* | 3 | 0.28 ± 0.01* | 4 | ||||||
| T1491D | 3.41 ± 0.16* | 5 | 0.95 ± 0.06* | 5 | 0.39 ± 0.01* | 5 | ||||||
| T1491M | 2.32 ± 0.11* | 4 | 0.63 ± 0.03* | 4 | 0.27 ± 0.01* | 4 | ||||||
| T1491V | 2.74 ± 0.11* | 3 | 0.71 ± 0.01* | 3 | 0.28 ± 0.01* | 3 | ||||||
| Y1497Q | 8.09 ± 0.36* | 3 | 1.66 ± 0.06* | 3 | 0.71 ± 0.10 | 3 | ||||||
| Y1498Q | 8.27 ± 0.61* | 3 | 1.44 ± 0.12* | 3 | 0.63 ± 0.10 | 3 | ||||||
| YY1497/8QQ | 3.39 ± 0.15* | 3 | 0.84 ± 0.01* | 3 | 0.36 ± 0.02* | 3 | ||||||
| MVF1523-5QQQ | 11.76 ± 0.67* | 3 | 2.04 ± 0.06 | 3 | 0.76 ± 0.05 | 3 | ||||||
| WT | 17.66 ± 1.42 | 4 | 2.51 ± 0.22 | 4 | 0.72 ± 0.03 | 4 |
The time course of recovery from inactivation at the potentials indicated was measured after a 15-ms depolarization to 0 mV and fitted with an equation including a single exponential component and a delay (see materials and methods).
*
Significantly different from WT (P < 0.05)