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. 2003 Apr;121(4):261–276. doi: 10.1085/jgp.200208771

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Copyright © 2003, The Rockefeller University Press

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Figure 2. — Effects of substituting aspartate in M2 of ROMK1 on inward rectification. (A) Voltage pulse protocol. (B-F) Currents of ROMK1 and four mutants containing Asp at various positions in M2. (G) Absolute ratio of steady-state currents (mean ± SEM; n = 5–12) at 80 and at −80 mV for wild-type and Asp-substituted channels, plotted against the M2 sequence.