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Composition of the Internal Recording Solutions
| Solution | A | B | C | D | E | F | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Na4-DM-nitrophen | 10 | 10 | 10 | 10 | 10 | 10 | ||||||
| CaCl2 | 8 | 8 | 10 | 10 | 10 | 8 | ||||||
| MgCl2 | 4 | 4 | 0 | 0 | 0 | 4 | ||||||
| LiCl | 0 | 0 | 0 | 0 | 40 | 0 | ||||||
| Na2-ATP | 10 | 0 | 0 | 0 | 0 | 10 | ||||||
| Li4-ATP-γ-S | 0 | 10 | 2 | 0 | 0 | 0 | ||||||
| K2-DTPA | 0 | 0 | 5 | 5 | 5 | 0 | ||||||
| GTP | 0.5 | 0.5 | 0.5 | 0 | 0.5 | 0 | ||||||
| Furaptra | 1 | 1 | 1 | 1 | 1 | 1 | ||||||
| Cs-glutamate | 0 | 0 | 0 | 62 | 0 | 55 | ||||||
| Cs-gluconate | 19 | 21 | 18 | 0 | 25 | 0 | ||||||
| Cs-HEPES | 56 | 62 | 54 | 31 | 75 | 28 | ||||||
| TEA-Cl | 4.9 | 5.5 | 4.8 | 6.24 | 6.6 | 5.54 | ||||||
| Free [Ca2+] | ≈250 nM | ≈250 nM | ≈0 | ≈0 | ≈0 | ≈250 nM | ||||||
| [MgATP] | ≈2 mM | ≈2 mM | ≈0 | ≈0 | ≈0 | ≈2 mM |
