TABLE I.
Screening for Inward Rectification Properties of Mutants
| No alteration of rectification | No functional currents | |
|---|---|---|
| N-cytoplasmic region | G65R, A70R, A70K, A70S, R74A R80Q | C54S, D71E, D78E |
| Pore region | A91T, V93I, V93T, F163V, I166V, G168T, I176T | S136F |
| C-cytoplasmic region | K223Q, I201V, L231E, S238K, E293Q, A306S, R325E, E333S, D340K, E349K, K362E, E387 | L217E, E303K, E303Q, G300V |
The amino acid sequence of Kir 2.1 was compared with that of sWIRK. To survey critical sites for inward rectification, these point mutants were made in the wild-type and D172N & E224G & E299Q triple mutant of Kir 2.1.