Table 1. siRNA-induced knock down/depletion of Dvl1, Dvl2, or Dvl3 in mammalian cells.
The cellular abundance of each Dvl isoform in mouse F9 cells was measured by immunoblotting. Blots were stained with Dvl isoform-specific antibodies. The abundance of the Dvl isoform in untreated cells was quantified and is set to a value of “1.0”. The results are presented as mean values ± S.E.M. from three or more separate experiments.
siRNA | Control | Dvl1 | Dvl2 | Dvl3 |
---|---|---|---|---|
Protein | ||||
Dvl1 | 1.00 ± 0.09 | 0.21 ± 0.04* | 1.09 ± 0.02 | 1.11 ± 0.09 |
Dvl2 | 1.00 ± 0.02 | 0.94 ± 0.03 | 0.14 ± 0.04* | 0.98 ± 0.02 |
Dvl3 | 1.00 ± 0.03 | 1.11 ± 0.08 | 1.07 ± 0.06 | 0.28 ± 0.03* |
, p < 0.001 for the difference from the values observed for untreated and control siRNA-treated cells.