Figure 1.
Inactivation of the RK gene. (A) Genomic structure of RK gene, targeting vector, and altered RK locus. The targeting vector contained 10 kb (long arm) of the 3′ downstream and 0.8 kb (short arm) of the 5′ upstream homologous sequence. The MC1neopA cassette (hatched bars) replaced the 2.7-kb BamHI fragment that contained the exon bearing the translation initiating codon (filled box). Restriction sites: B, BamHI; A, ApaI; K, KpnI. (B) Immunoblot analysis of RK from mice at 1 month of age; the analysis used a polyclonal antibody (8585) raised against the first 50 amino acids of bovine RK. The RK content of the hemizygous (+/−) retina was approximately 50% of that of the RK+/+. No detectable RK was found in the RK−/− retina.