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The results of PCR assays (using an independent ChIP sample) for 14 Oct4 binding sites predicted from the WGA array data are shown. Two different binding sites were assayed for the HoxA1, EVX1, and EXT1 genes. Primers specific to the NANOG promoter were used as a positive control and primers specific to the DHFR gene were used as a negative control. Enrichments above the negative control are considered confirmed Oct4 binding sites.
