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Chromatograms of the molar masses for the recombinant proteins (A) wt βB1, (B) N157D, and (C) Q146E. Filled diamonds represent the molar masses determined for each eluting slice. The line represents the refractive index signal; all refractive index tracings are normalized to one another. The concentration of each protein was 10 mg/mL in a 50-μL sample.
