Table 1.
Systematic variation from consensus DR1-type PPRE
| Percent binding strength | PPRE position | ||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | |
| PPARα | |||||||||||||
| Consensus (90-100) | A/G | G | G | T | A/C | A | A | A | G | G | T | C | A |
| Class I (60-90) | T | C | G | T | G | T | C/G | A/G | G | ||||
| Class II (30-60) | C | T | A/T | A/C/G | T | T | C/G | C | A/C/T | T | C/T | ||
| Class III (0-30) | A/C | C/G | T | A/C | A | ||||||||
| PPARγ | |||||||||||||
| Consensus (90-100) | A/G | G | G | T | C/G | A | A | A | G | G | T | C | A |
| Class I (60-90) | C/G | A/T | T | G | T | C/G | A/G/T | G | |||||
| Class II (30-60) | C/T | A/T | T | A | C | C | A/C/T | C/T | |||||
| Class III (0-30) | C | A/C | C/G/T | G | T | A/C | A | ||||||
| PPARβ/δ | |||||||||||||
| Consensus (90-100) | A/G | G | G | T | C | A | A | A | G | G | T | C | A |
| Class I (60-90) | C/G | G/T | T | G | T | G/T | |||||||
| Class II (30-60) | C | A/T | T | A | A | A/T | C/G | A | G/C/T | ||||
| Class III (0-30) | T | C | A/C | C/G/T | C/G | C/T | C | A/C | A | ||||
The binding strengths of in vitro translated PPAR-RXR heterodimers to 39 systematic variations of the DR1-type consensus PPRE AGGTCAAAGGTCA were determined by gelshift assays in reference to this consensus PPRE. Based on their average binding strength, all variations are sorted into three classes.