Table 1.
Human postmortem midbrain samples—characteristics and gene-expression
| BBC | Sex, Age/a | CERAD/ Braak | PMI/h RIN | cDNA amount per SN neuron (pg) (n/x) | Disease state | ||||
|---|---|---|---|---|---|---|---|---|---|
| TH | ENO2 | α-SYN | |||||||
| PD | SN RZ 238 | f, 79 | 0, II | 17 | 6.0 | 2.03 ± 0.40 (8/8) | 1.41 ± 0.19 (8/8) | 4.62 ± 0.66 (8/8) | PD |
| SN RZ 239 | m, 79 | 0, 0 | 23 | 7.1 | 1.40 ± 0.40 (8/8) | 2.23 ± 0.55 (8/8) | 3.23 ± 0.96 (8/8) | PD, AD, cardiac infarction, lung cancer | |
| SN RZ 224 | m, 80 | 0, II | 7 | 7.2 | 1.01 ± 0.18 (8/8) | 1.25 ± 0.30 (8/8) | 3.01 ± 0.70 (8/8) | PD, multisystem atrophy | |
| SN RZ 389 | f, 73 | 0, V | 11 | 7.3 | 1.76 ± 0.31 (8/8) | 2.01 ± 0.45 (8/8) | 2.81 ± 0.45 (8/8) | PD, ovary cancer, Sudeck's atrophy | |
| Control | SN 323/01 | f, 69 | A, I | 14 | 4.9 | 0.27 ± 0.08 (8/8) | 0.17 ± 0.08 (8/8) | 1.11 ± 0.29 (8/8) | diabetes, hypertonia, pneumonia, ARDS |
| SN 2/02 | m, 62 | 0, I | 31 | 6.1 | 0.38 ± 0.14 (7/8) | 0.27 ± 0.07 (6/8) | 1.20 ± 0.48 (7/8) | hypertonia, cardiac infarction | |
| SN 15/02 | m, 72 | 0, II | 6 | 5.2 | 0.08 ± 0.02 (8/8) | 0.06 ± 0.02 (8/8) | 0.38 ± 0.06 (8/8) | CMV, diabetes, kidney transplant, nephritis | |
| BBC 2-12 | f, 75 | 0, I | 24 | 4.8 | 0.06 ± 0.02 (8/8) | 0.04 ± 0.01 (5/8) | 0.11 ± 0.02 (8/8) | atherosclerosis | |
| BBC 3-12 | m, 63 | 0, 0 | 24 | 5.9 | 0.07 ± 0.02 (8/8) | 0.12 ± 0.12 (2/8) | 0.15 ± 0.04 (8/8) | liver cancer, hepatorenal syndrome | |
German BrainNet case number (BBC), sex/age, disease states classified according to the Consortium to establish a registry for Alzheimer's disease (CERAD) and according to the classification of Braak and Braak 1991 (Braak), postmortem interval (PMI), RNA integrity number (RIN) and cDNA amount per SN neuron (as pg-equivalents of total cDNA, derived from human SN-tissue) for the genes tyrosine hydroxylase (TH), neuron-specific enolase (ENO2) and α-synuclein (α-SYN) (mean ± SEM) are given for all analyzed PD and control brain tissues. Number of analyzed pools (x) and number of pools which expressed the tested gene (n) are indicated for each brain.