Table 2. 17β-E2 does not upregulate manganese superoxide dismutase activity after 6 h.
|
Time |
U/ml |
Time |
U/mg protein |
||
|
Control |
(+) 17b E2 |
Control |
(+) 17b E2 |
||
| 90 min |
20.4±4.4 |
91±10.8* |
90 min |
11.6±3 |
62±8* |
| 6 h | 45±12 | 49±6 | 6 h | 30±8 | 33±5 |
A second independent experiment with a fresh cell population was repeated over an extended time course with HLE-B3 cells maintained in 0.5% serum and exposed to 1 μM 17β-E2 for 90 min and 6 h (n=8, from two individual cell populations±SEM). The control represents cells that were not treated with 17β-E2. Note the significant increase in activity (asterisk) detected with the 90 min incubation treated with 17β-E2 and the return to equivalent control activity levels by 6 h of treatment (compare 6 h control versus 6 h +17β-E2). n=8; the asterisk indicates statistical significance as determined by Student’s t-test.