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. Author manuscript; available in PMC: 2009 Apr 25.
Published in final edited form as: Virology. 2008 Jan 22;374(1):1–10. doi: 10.1016/j.virol.2007.11.023

Figure 2. HSV-2 UL13 autophosphorylates.

Figure 2

Empty vector pSG5 (Mock), UL13-HA, and K176A-HA were translated in vitro, isolated by immunoprecipitation, and subjected to an in vitro kinase assay. Proteins were resolved by SDS-PAGE and (A) HA-tagged UL13 and K176A were detected by western blot using HA-specific MAb 3F-10 and inset and (B) 32P-labeled protein was detected by autoradiography of the western blot and inset. Asterisk denotes slower mobility isoform of UL13.