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Kinetic parameters of wild type and mutant QFR
All assays were done at 30 °C. Fumarate reduction with methyl viologen (pH 7.0) and succinate oxidation with potassium ferricyanide (pH 8.0) were performed as described under “Experimental Procedures.” ND, not determined.
|
Fumarate reduction |
Succinate oxidation |
||||
|---|---|---|---|---|---|
| kcat | Kmfum | kcat | Kmsucc | KiOAA | |
| s−1 | mm | s−1 | mm | μm | |
| WT-QFR | 340 | 0.02 | 30 | 0.55 | 0.3 |
| AcT-QFR | 0.4 | 0.8 | <0.03 | ND | ND |
| HingeT-QFR | 26 | 0.6 | 1.3 | 2.7 | 40 |
