Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1992 Dec;66(12):7239–7244. doi: 10.1128/jvi.66.12.7239-7244.1992

Characterization of reticuloendotheliosis virus-transformed avian T-lymphoblastoid cell lines infected with Marek's disease virus.

W D Pratt 1, R W Morgan 1, K A Schat 1
PMCID: PMC240427  PMID: 1279200

Abstract

The expression of Marek's disease virus (MDV) transcripts and protein products was investigated in reticuloendotheliosis virus-transformed avian T-lymphoblastoid cell line RECC-CU91, which was superinfected with MDV. The presence of MDV in the superinfected cell line, renamed RECC-CU210, was demonstrated by Southern hybridization with 32P-labeled BamHI-H and -B fragments of the BamHI MDV DNA library. Examination of RECC-CU210 for the expression of MDV-specific RNA transcripts encoded by the internal repeat long (IRL), internal repeat short (IRS), and unique short (US) regions of the MDV genome revealed two small transcripts of 0.6 and 0.7 kb. These transcripts were mapped to the IRL and IRS regions, respectively. In contrast, RECC-CU211, which was developed through transfection of CU210 with the BamHI-A fragment of MDV, expressed an additional nine transcripts from the IRL, IRS, and US regions. CU211 but not CU210 also expressed a complex of polypeptides of 40, 38, and 24 kDa, identified by monoclonal antibodies as MDV-specific phosphoproteins. The 38-kDa phosphoprotein is likely to be pp38, an early viral protein that maps within the IRL region of the MDV genome. These findings suggest that genes located within the transfected BamHI-A fragment transactivated a number of genes located in the IRL region of the MDV genome.

Full text

PDF
7239

Images in this article

7241Image
on p.7241
7241Image
on p.7241
7242Image
on p.7242
7242Image
on p.7242

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson A. S., Francesconi A., Morgan R. W. Complete nucleotide sequence of the Marek's disease virus ICP4 gene. Virology. 1992 Aug;189(2):657–667. doi: 10.1016/0042-6822(92)90589-h. [DOI] [PubMed] [Google Scholar]
  2. Boshart M., Weber F., Jahn G., Dorsch-Häsler K., Fleckenstein B., Schaffner W. A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus. Cell. 1985 Jun;41(2):521–530. doi: 10.1016/s0092-8674(85)80025-8. [DOI] [PubMed] [Google Scholar]
  3. Buckmaster A. E., Scott S. D., Sanderson M. J., Boursnell M. E., Ross N. L., Binns M. M. Gene sequence and mapping data from Marek's disease virus and herpesvirus of turkeys: implications for herpesvirus classification. J Gen Virol. 1988 Aug;69(Pt 8):2033–2042. doi: 10.1099/0022-1317-69-8-2033. [DOI] [PubMed] [Google Scholar]
  4. Calnek B. W., Shek W. R., Schat K. A. Spontaneous and induced herpesvirus genome expression in Marek's disease tumor cell lines. Infect Immun. 1981 Nov;34(2):483–491. doi: 10.1128/iai.34.2.483-491.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cebrian J., Kaschka-Dierich C., Berthelot N., Sheldrick P. Inverted repeat nucleotide sequences in the genomes of Marek disease virus and the herpesvirus of the turkey. Proc Natl Acad Sci U S A. 1982 Jan;79(2):555–558. doi: 10.1073/pnas.79.2.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chandratilleke D., O'Connell P., Schat K. A. Characterization of proteins of chicken infectious anemia virus with monoclonal antibodies. Avian Dis. 1991 Oct-Dec;35(4):854–862. [PubMed] [Google Scholar]
  7. Chen X. B., Sondermeijer P. J., Velicer L. F. Identification of a unique Marek's disease virus gene which encodes a 38-kilodalton phosphoprotein and is expressed in both lytically infected cells and latently infected lymphoblastoid tumor cells. J Virol. 1992 Jan;66(1):85–94. doi: 10.1128/jvi.66.1.85-94.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Colbère-Garapin F., Horodniceanu F., Kourilsky P., Garapin A. C. A new dominant hybrid selective marker for higher eukaryotic cells. J Mol Biol. 1981 Jul 25;150(1):1–14. doi: 10.1016/0022-2836(81)90321-1. [DOI] [PubMed] [Google Scholar]
  9. Croen K. D., Ostrove J. M., Dragovic L. J., Straus S. E. Patterns of gene expression and sites of latency in human nerve ganglia are different for varicella-zoster and herpes simplex viruses. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9773–9777. doi: 10.1073/pnas.85.24.9773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Cui Z. Z., Lee L. F., Liu J. L., Kung H. J. Structural analysis and transcriptional mapping of the Marek's disease virus gene encoding pp38, an antigen associated with transformed cells. J Virol. 1991 Dec;65(12):6509–6515. doi: 10.1128/jvi.65.12.6509-6515.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fukuchi K., Sudo M., Lee Y. S., Tanaka A., Nonoyama M. Structure of Marek's disease virus DNA: detailed restriction enzyme map. J Virol. 1984 Jul;51(1):102–109. doi: 10.1128/jvi.51.1.102-109.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ikuta K., Nakajima K., Naito M., Ann S. H., Ueda S., Kato S., Hirai K. Identification of Marek's disease virus-specific antigens in Marek's disease lymphoblastoid cell lines using monoclonal antibody against virus-specific phosphorylated polypeptides. Int J Cancer. 1985 Feb 15;35(2):257–264. doi: 10.1002/ijc.2910350219. [DOI] [PubMed] [Google Scholar]
  13. Kanamori A., Ikuta K., Ueda S., Kato S., Hirai K. Methylation of Marek's disease virus DNA in chicken T-lymphoblastoid cell lines. J Gen Virol. 1987 May;68(Pt 5):1485–1490. doi: 10.1099/0022-1317-68-5-1485. [DOI] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Maray T., Malkinson M., Becker Y. RNA transcripts of Marek's disease virus (MDV) serotype-1 in infected and transformed cells. Virus Genes. 1988 Oct;2(1):49–68. doi: 10.1007/BF00569736. [DOI] [PubMed] [Google Scholar]
  16. Priola S. A., Gustafson D. P., Wagner E. K., Stevens J. G. A major portion of the latent pseudorabies virus genome is transcribed in trigeminal ganglia of pigs. J Virol. 1990 Oct;64(10):4755–4760. doi: 10.1128/jvi.64.10.4755-4760.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ross L. J., Binns M. M., Pastorek J. DNA sequence and organization of genes in a 5.5 kbp EcoRI fragment mapping in the short unique segment of Marek's disease virus (strain RB1B). J Gen Virol. 1991 Apr;72(Pt 4):949–954. doi: 10.1099/0022-1317-72-4-949. [DOI] [PubMed] [Google Scholar]
  18. Schat K. A., Buckmaster A., Ross L. J. Partial transcription map of Marek's disease herpesvirus in lytically infected cells and lymphoblastoid cell lines. Int J Cancer. 1989 Jul 15;44(1):101–109. doi: 10.1002/ijc.2910440119. [DOI] [PubMed] [Google Scholar]
  19. Schat K. A., Chen C. L., Shek W. R., Calnek B. W. Surface antigens on Marek's disease lymphoblastoid tumor cell lines. J Natl Cancer Inst. 1982 Sep;69(3):715–720. [PubMed] [Google Scholar]
  20. Schat K. A., Pratt W. D., Morgan R., Weinstock D., Calnek B. W. Stable transfection of reticuloendotheliosis virus-transformed lymphoblastoid cell lines. Avian Dis. 1992 Apr-Jun;36(2):432–439. [PubMed] [Google Scholar]
  21. Silva R. F., Lee L. F. Monoclonal antibody-mediated immunoprecipitation of proteins from cells infected with Marek's disease virus or turkey herpesvirus. Virology. 1984 Jul 30;136(2):307–320. doi: 10.1016/0042-6822(84)90167-3. [DOI] [PubMed] [Google Scholar]
  22. Sugaya K., Bradley G., Nonoyama M., Tanaka A. Latent transcripts of Marek's disease virus are clustered in the short and long repeat regions. J Virol. 1990 Dec;64(12):5773–5782. doi: 10.1128/jvi.64.12.5773-5782.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wong S. W., Schaffer P. A. Elements in the transcriptional regulatory region flanking herpes simplex virus type 1 oriS stimulate origin function. J Virol. 1991 May;65(5):2601–2611. doi: 10.1128/jvi.65.5.2601-2611.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES