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. 1984 Oct;48(4):726–731. doi: 10.1128/aem.48.4.726-731.1984

Construction of plasmid cloning vectors for lactic streptococci which also replicate in Bacillus subtilis and Escherichia coli.

J Kok, J M van der Vossen, G Venema
PMCID: PMC241602  PMID: 6095756

Abstract

The cryptic Streptococcus cremoris Wg2 plasmid pWV01 (1.5 megadaltons) was genetically marked with the chloramphenicol resistance (Cmr) gene from pC194. The recombinant plasmid (pGK1, 2.4 megadaltons) replicated and expressed Cmr in Bacillus subtilis. From this plasmid an insertion-inactivation vector was constructed by inserting the erythromycin resistance (Emr) gene from pE194 cop-6. This plasmid (pGK12, 2.9 megadaltons) contained a unique BclI site in the Emr gene and unique ClaI and HpaII sites outside both resistance genes. It was stably maintained in B. subtilis at a copy number of approximately 5. pGK12 also transformed Escherichia coli competent cells to Cmr and Emr. The copy number in E. coli was about 60. Moreover, pGK12 transformed protoplasts of Streptococcus lactis. In this host both resistance genes are expressed. pGK12 is stably maintained in S. lactis at a copy number of 3.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barany F., Boeke J. D., Tomasz A. Staphylococcal plasmids that replicate and express erythromycin resistance in both Streptococcus pneumoniae and Escherichia coli. Proc Natl Acad Sci U S A. 1982 May;79(9):2991–2995. doi: 10.1073/pnas.79.9.2991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bron S., Venema G. Ultraviolet inactivation and excision-repair in Bacillus subtilis. I. Construction and characterization of a transformable eightfold auxotrophic strain and two ultraviolet-sensitive derivatives. Mutat Res. 1972 May;15(1):1–10. doi: 10.1016/0027-5107(72)90086-3. [DOI] [PubMed] [Google Scholar]
  3. Chang S., Cohen S. N. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol Gen Genet. 1979 Jan 5;168(1):111–115. doi: 10.1007/BF00267940. [DOI] [PubMed] [Google Scholar]
  4. Davies F. L., Gasson M. J. Reviews of the progress of dairy science: genetics of lactic acid bacteria. J Dairy Res. 1981 Jun;48(2):363–376. doi: 10.1017/s0022029900021798. [DOI] [PubMed] [Google Scholar]
  5. Espinosa M., Lopez P., Perez-Ureña M. T., Lacks S. A. Interspecific plasmid transfer between Streptococcus pneumoniae and Bacillus subtilis. Mol Gen Genet. 1982;188(2):195–201. doi: 10.1007/BF00332675. [DOI] [PubMed] [Google Scholar]
  6. Gasson M. J. Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. J Bacteriol. 1983 Apr;154(1):1–9. doi: 10.1128/jb.154.1.1-9.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goze A., Ehrlich S. D. Replication of plasmids from Staphylococcus aureus in Escherichia coli. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7333–7337. doi: 10.1073/pnas.77.12.7333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hardy K., Haefeli C. Expression in Escherichia coli of a staphylococcal gene for resistance to macrolide, lincosamide, and streptogramin type B antibiotics. J Bacteriol. 1982 Oct;152(1):524–526. doi: 10.1128/jb.152.1.524-526.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hardy K., Stahl S., Küpper H. Production in B. subtilis of hepatitis B core antigen and a major antigen of foot and mouth disease virus. Nature. 1981 Oct 8;293(5832):481–483. doi: 10.1038/293481a0. [DOI] [PubMed] [Google Scholar]
  10. Heyting C., Talen J. L., Weijers P. J., Borst P. Fine structure of the 21S ribosomal RNA region on yeast mitochondrial DNA. II. The organization of sequences in petite mitochondrial DNAs carrying genetic markers from the 21S region. Mol Gen Genet. 1979 Jan 11;168(3):251–277. doi: 10.1007/BF00271497. [DOI] [PubMed] [Google Scholar]
  11. Hohn B. In vitro packaging of lambda and cosmid DNA. Methods Enzymol. 1979;68:299–309. doi: 10.1016/0076-6879(79)68021-7. [DOI] [PubMed] [Google Scholar]
  12. Horinouchi S., Weisblum B. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. J Bacteriol. 1982 May;150(2):815–825. doi: 10.1128/jb.150.2.815-825.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Horinouchi S., Weisblum B. Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibodies. J Bacteriol. 1982 May;150(2):804–814. doi: 10.1128/jb.150.2.804-814.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Horinouchi S., Weisblum B. Posttranscriptional modification of mRNA conformation: mechanism that regulates erythromycin-induced resistance. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7079–7083. doi: 10.1073/pnas.77.12.7079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Iordanescu S., Surdeanu M., Della Latta P., Novick R. Incompatibility and molecular relationships between small Staphylococcal plasmids carrying the same resistance marker. Plasmid. 1978 Sep;1(4):468–479. doi: 10.1016/0147-619x(78)90005-7. [DOI] [PubMed] [Google Scholar]
  16. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Jarvis A. W., Jarvis B. D. Deoxyribonucleic Acid homology among lactic streptococci. Appl Environ Microbiol. 1981 Jan;41(1):77–83. doi: 10.1128/aem.41.1.77-83.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Klaenhammer T. R., McKay L. L., Baldwin K. A. Improved lysis of group N streptococci for isolation and rapid characterization of plasmid deoxyribonucleic acid. Appl Environ Microbiol. 1978 Mar;35(3):592–600. doi: 10.1128/aem.35.3.592-600.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Klotz L. C., Zimm B. H. Size of DNA determined by viscoelastic measurements: results on bacteriophages, Bacillus subtilis and Escherichia coli. J Mol Biol. 1972 Dec 30;72(3):779–800. doi: 10.1016/0022-2836(72)90191-x. [DOI] [PubMed] [Google Scholar]
  20. Kondo J. K., McKay L. L. Transformation of Streptococcus lactis Protoplasts by Plasmid DNA. Appl Environ Microbiol. 1982 May;43(5):1213–1215. doi: 10.1128/aem.43.5.1213-1215.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  22. McKay L. L., Baldwin K. A., Walsh P. M. Conjugal transfer of genetic information in group N streptococci. Appl Environ Microbiol. 1980 Jul;40(1):84–89. doi: 10.1128/aem.40.1.84-91.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. McKay L. L., Cords B. R., Baldwin K. A. Transduction of lactose metabolism in Streptococcus lactis C2. J Bacteriol. 1973 Sep;115(3):810–815. doi: 10.1128/jb.115.3.810-815.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rottländer E., Trautner T. A. Genetic and transfection studies with B, subtilis phage SP 50. I. Phage mutants with restricted growth on B. subtilis strain 168. Mol Gen Genet. 1970;108(1):47–60. doi: 10.1007/BF00343184. [DOI] [PubMed] [Google Scholar]
  25. Scheer-Abramowitz J., Gryczan T. J., Dubnau D. Origin and mode of replication of plasmids pE194 and pUB110. Plasmid. 1981 Jul;6(1):67–77. doi: 10.1016/0147-619x(81)90054-8. [DOI] [PubMed] [Google Scholar]
  26. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  27. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Terzaghi B. E., Sandine W. E. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975 Jun;29(6):807–813. doi: 10.1128/am.29.6.807-813.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Vosman B., Venema G. Introduction of a Streptococcus cremoris plasmid in Bacillus subtilis. J Bacteriol. 1983 Nov;156(2):920–921. doi: 10.1128/jb.156.2.920-921.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Weisblum B., Graham M. Y., Gryczan T., Dubnau D. Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194. J Bacteriol. 1979 Jan;137(1):635–643. doi: 10.1128/jb.137.1.635-643.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. de Vos W. M., Venema G., Canosi U., Trautner T. A. Plasmid transformation in Bacillus subtilis: fate of plasmid DNA. Mol Gen Genet. 1981;181(4):424–433. doi: 10.1007/BF00428731. [DOI] [PubMed] [Google Scholar]

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