FIG. 2.
CHD8 is an ATP-dependent remodeling factor. (A) Recombinant CHD8 or CHD8(K842R) was assayed for increased restriction enzyme accessibility of mononucleosomes. (Inset) Representative data. The top band is the uncut template, and the bottom band is the resulting cut fragment. (B) Time course of restriction enzyme cleavage in the presence and absence of CHD8. (C) Recombinant CHD8 was assayed for nucleosome sliding activity with nucleosomes reconstituted on a DNA template without a nucleosome positioning sequence (lanes 3 to 6). Also included were mobility standards reconstituted on DNA templates with a centrally (Mid) or terminally (End) located nucleosome positioning sequence (lanes 1 and 2, respectively).