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. 2008 May 22;27(12):1747–1757. doi: 10.1038/emboj.2008.96

Figure 2.

Figure 2

Analysis of VhlhΔ/Δ, PtenΔ/Δ and VhlhΔ/ΔPtenΔ/Δ mice. (A) PCR analysis of recombination at the Vhlh and Pten loci in mice with combinations of the Ksp1.3-Cre transgene and Vhlh and Pten floxed and wild-type alleles. The positions of the bands representing the Vhlh and Pten floxed (fl), wild type (+) and recombined (Δ) alleles are indicated. (B) Comparison of the size and external morphology of WT and VhlhΔ/ΔPtenΔ/Δ kidneys (bar=1 cm). (C) Mass of kidneys from WT, VhlhΔ/Δ, PtenΔ/Δ and VhlhΔ/ΔPtenΔ/Δ mice (n⩾9, aged 8–21 weeks). *P<0.01, Student's t-test. (D–G) Histological appearance of renal cortex from littermate 8-week-old WT, VhlhΔ/Δ, PtenΔ/Δ and VhlhΔ/ΔPtenΔ/Δ mice (bar=100 μm). Examples of (H) simple and (I) atypical cysts, (J) clear cell morphology (arrowheads) and (K) a region of squamous metaplasia (SM) characterised by keratinocyte-like cells surrounding a core of keratin (pink staining), in VhlhΔ/ΔPtenΔ/Δ renal cortex. Immunofluorescence staining for (L) the collecting duct marker FITC-tagged Dolichos biflorus agglutinin and (M) the distal tubule marker NaCl cotransporter in cysts in VhlhΔ/ΔPtenΔ/Δ renal cortex. Bars in (H–M)=10 μm.