Figure 3.
An AP1 site is responsible for transactivation of the GPR30–5′-flanking region induced by EGF in SkBr3 and BT20 breast cancer cells. A, AP1 (GPR30AP1mut) and SP1 (GPR30SP1mut) mutations generated within the GPR30–5′-flanking region. B and C, Cells were transfected with the reporter plasmids described in A and treated with 50 ng/ml EGF. The luciferase activities were normalized to the internal transfection control and values of cells receiving vehicle (−) were set as 1-fold induction upon which the activity induced by treatments was calculated. Each data point represents the mean ± sd of three independent experiments performed in triplicate. ○, •, P < 0.05, for cells receiving vehicle (−) vs. treatment.