FIG. 3.
SLS hemolytic activity is repressed by CcpA. WT MGAS5005 (empty vector), ΔccpA mutant (empty vector), and complemented ΔccpA (ccpA) cells were grown in THY broth supplemented with 10% heat-inactivated horse serum, and supernatant samples were isolated at different time points for analysis of SLS activity. Data are presented as average percentages of hemolysis (solid lines) for three independent experiments, with standard error bars. The growth for each strain normalized to growth of WT MGAS5005 is shown in Klett units for one representative experiment (dashed lines).