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. 1969 Mar;97(3):1291–1297. doi: 10.1128/jb.97.3.1291-1297.1969

Nitrate Reductase Complex of Escherichia coli K-12: Isolation and Characterization of Mutants Unable to Reduce Nitrate

José Ruiz-Herrera a,1, Michael K Showe a,2, J A DeMoss a
PMCID: PMC249846  PMID: 4887509

Abstract

Thirty-eight mutants unable to reduce nitrate were isolated from Escherichia coli and characterized biochemically and genetically. All of the mutants exhibited reduced or insignificant levels of formate dehydrogenase, nitrate reductase, or various combinations of these activities and cytochrome b1 under conditions which resulted in the production of high levels of these activities by the wild-type parental strains. Most of the mutants reverted readily to wild type, and all mapped within a restricted region on the chromosome linked to the tryptophan genes. It was proposed that nitrate reduction in E. coli was catalyzed exclusively by an organized complex containing formate dehydrogenase, cytochrome b1, and nitrate reductase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMES B. N., MARTIN R. G. BIOCHEMICAL ASPECTS OF GENETICS: THE OPERON. Annu Rev Biochem. 1964;33:235–258. doi: 10.1146/annurev.bi.33.070164.001315. [DOI] [PubMed] [Google Scholar]
  2. Azoulay E., Puig J., Pichinoty F. Alteration of respiratory particles by mutation in Escherichia coli K 12. Biochem Biophys Res Commun. 1967 Apr 20;27(2):270–274. doi: 10.1016/s0006-291x(67)80073-1. [DOI] [PubMed] [Google Scholar]
  3. ELLS H. A. A colorimetric method for the assay of soluble succinic dehydrogenase and pyridinenucleotide-linked dehydrogenases. Arch Biochem Biophys. 1959 Dec;85:561–562. doi: 10.1016/0003-9861(59)90527-2. [DOI] [PubMed] [Google Scholar]
  4. Giles N. H., Case M. E., Partridge C. W., Ahmed S. I. A gene cluster in Nuerospora crassa coding for an aggregate of five aromatic synthetic enzymes. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1453–1460. doi: 10.1073/pnas.58.4.1453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kemp J. D., Atkinson D. E. Nitrite reductase of Escherichia coli specific for reduced nicotinamide adenine dinucleotide. J Bacteriol. 1966 Sep;92(3):628–634. doi: 10.1128/jb.92.3.628-634.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. O'Hara J., Gray C. T. Defects in formate hydrogenlyase in nitrate-negative mutants of Escherichia coli. Biochem Biophys Res Commun. 1967 Sep 27;28(6):951–957. doi: 10.1016/0006-291x(67)90072-1. [DOI] [PubMed] [Google Scholar]
  7. Piéchaud M., Puig J., Pichinoty F., Azoulay E., Le Minor L. Mutations affectant la nitrate-réductase A et d'autres enzymes bactériennes d'oxydoréduction. Ann Inst Pasteur (Paris) 1967 Jan;112(1):24–37. [PubMed] [Google Scholar]
  8. Showe M. K., DeMoss J. A. Localization and regulation of synthesis of nitrate reductase in Escherichia coli. J Bacteriol. 1968 Apr;95(4):1305–1313. doi: 10.1128/jb.95.4.1305-1313.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sypherd P. S., Strauss N. CHLORAMPHENICOL-PROMOTED REPRESSION OF beta-GALACTOSIDASE SYNTHESIS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1963 Mar;49(3):400–407. doi: 10.1073/pnas.49.3.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Venables W. A., Wimpenny J. W., Cole J. A. Enzymic properties of a mutant of Escherichia coli K12 lacking nitrate reductase. Arch Mikrobiol. 1968;63(2):117–121. doi: 10.1007/BF00412166. [DOI] [PubMed] [Google Scholar]
  11. Wimpenny J. W., Cole J. A. The regulation of metabolism in facultative bacteria. 3. The effect of nitrate. Biochim Biophys Acta. 1967 Oct 9;148(1):233–242. doi: 10.1016/0304-4165(67)90298-x. [DOI] [PubMed] [Google Scholar]

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