TABLE 1.
qRT-PCR primersa
| Gene | Primer sequence |
|---|---|
| NS1 | GAATGGCATTGTGTTTGTGC |
| TGGCATTGTTGTGAAATTGG | |
| N | TGCAGGGCAAGTGATGTTAC |
| TTCCATTTCTGCTTGCACAC | |
| P | GGCAAGACTCAGGAATGAGG |
| TCCCTTCCAACAGGTTGTTC | |
| M | AATGCCCAGCAAATTTACCA |
| GCCTTGATTTCACAGGGTGT | |
| SH | CCAATCTGATGGCACAAAAC |
| GCTTGCATGGTGAGATGTTG | |
| G | AAGTCAACCCTGCAATCCAC |
| TTTGTTTTGGCGTTGTTTTG | |
| F | TAGGAGCCATTGTGTCATGC |
| ATCGCACCCGTTAGAAAATG | |
| M2 | CCCATGCACTGCTTGTAAGA |
| CCAACTCTGCAGCTCCACTT | |
| L | TCCTGCTACAGATGCAACCA |
| ACAGGCAATTCAGCATCACA | |
| GAPDH | GGCCTCCAAGGAGTAAGACC |
| AGGGGAGATTCAGTGTGGTG | |
| IFN-β | TGCTCTGGCACAACAGGTAG |
| TGGAGAAGCACAACAGGAGA |
a
The GenBank accession numbers for RSV, GAPDH, and IFN-β sequences are M74568, NM_002046, and NM_002176, respectively. For each gene, the top sequence is the sense strand and the bottom sequence is the antisense strand. All primers are written 5′ to 3′.