Figure 3. Chitin-induced eosinophil recruitment is independent of Stat6 and Rag, but is dependent on BLT1.
a, Stat6−/ −×4get and 4get mice received chitin or PBS, and the lungs were analyzed for eosinophils. b, Rag−/ − and wild-type mice received chitin or PBS, and lungs were analyzed for eosinophils. Eosinophils were identified as DAPI−, FSCIo, CD4−, Siglec F+, SSChi. c, Transwell analysis of eosinophil recruitment by RAW267.4 macrophages incubated with vehicle, chitin, or AMCase-treated chitin. Where indicated, macrophages were pretreated with vehicle or MK886 (10 μM) for 10 min at 37°C prior to addition of chitin. d, BLT1−/ − mice and WT C57BL/6 mice received intranasal chitin. e, 4get mice were treated intraperitoneally with clodronate liposomes (CLL) or PBS on days 0 and 3. On day 4 mice received chitin i.p. and were analyzed 6 hrs later. f, Kit W-sh or wild-type controls received chitin intranasally. P-values were determined using Student’s t-test. Error bars represent standard deviations. * p<0.05, ** p<0.01 and *** p<0.001 Numbers in FACS plots are percentages of gated cells from total live cells. Experiments represent 2–3 independent experiments with n=3–4 per group.