Table 1.
Rate of high quality (Phred score ≥ 20) artifactual nucleotide substitutions at least 2 bp outside homopolymer tracts and excluding primer sequences observed in 12,700 reads from the control locus
| Substitution | Number* | Number bases sequenced* | Observed error rate |
|---|---|---|---|
| T → C | 1073 | 852,624 | 1.2 × 10−3 |
| A → G | |||
| G → A | 504 | 1,373,928 | 3.7 × 10−4 |
| C → T | |||
| T → G | 128 | 852,624 | 1.5 × 10−4 |
| A → C | |||
| G → C | 174 | 1,373,928 | 1.3 × 10−4 |
| C → G | |||
| T → A | 71 | 852,624 | 8.3 × 10−5 |
| A → T | |||
| G → T | 85 | 1,373,928 | 6.2 × 10−5 |
| C → A | |||
| Total | 2035 | 2,226,552 | 9 × 10−4 |
*Excluding bases sequenced within 2 bp either side of a homopolymer tract and primer sequence.