Figure 3.
The phosphorylation of Exo1 is checkpoint dependent. All strains carried EXO1-myc∷HIS3. For each experiment, two different strains with the same genotype, indicated as A and B were examined. (A) cdc13-1 (DLY1529) and cdc13-1 rad24Δ (DLY2974 and DLY2975), cdc13-1 rad17Δ (DLY3844 and DLY3845), cdc13-1 rad9Δ (DLY3009 and DLY3010), cdc13-1 rad53Δ sml1Δ (DLY3073 and DLY3074), strains exponentially growing at 23°C, were grown for a further 6 h at 23 or 36°C. Western blots of TCA protein extracts were first probed with anti-Myc antibodies to detect Exo1-Myc. Membranes were stripped and reprobed with anti-Rad53 antibodies. (B) As in (A) but with cdc13-1 mec1Δ sml1Δ strains (DLY4146 and DLY3261) and cdc13-1 mec1Δ sml1Δ tel1Δ strains (DLY4115). (C) As in (A) but with cdc13-1 tel1Δ (DLY3002 and DLY3003), cdc13-1 chk1Δ (DLY3011 and DLY3012), cdc13-1 dun1Δ strains (DLY3077 and DLY3078). (D) As in (A) but with cdc13-1 Rad53KD strain (DLY3568 and DLY3569).