Fig. 1.
VPA facilitates reelin promoter demethylation. (A) Mice were pretreated for 7 days with vehicle (open bar) or MET (5.2 mmol/kg s. c. twice a day) (filled bar) to induce hypermethylation of the reelin promoter. (B) After MET treatment termination, VPA (0 to 2.0 mmol/kg s. c. twice a day for 3 days) induces a dose-related decrease of reelin promoter methylation (shaded bars). Reelin promoter methylation was measured 2 h after the last injection of VPA. ¥, depicted are the ratios between the amount of reelin promoter fragment immunoprecipitated with MeCP2 antibodies (MeCP2-ChIP) and the amount of the corresponding promoter fragment in the initial non immunoprecipitated extract (input). The data represent the mean ± SE of 3 to 5 mice. *, P < 0.01 when the effect of VPA is compared with the group with no VPA. (One-way ANOVA followed by Bonferroni comparison).