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. 2008 Jan 30;105(6):1943–1948. doi: 10.1073/pnas.0711734105

Fig. 3.

Fig. 3.

PPARγ1 phosphorylation is coupled to increased transcription of target genes. (A) Real-time PCR experiment of ADRP under a 6-h influence of EC16 or rosiglitazone. (B) Real-time PCR of FIAF expression influenced by EC16 or rosiglitazone for 6 h. (C) Expressional changes of IL-10 in the presence of both rosiglitazone and EC16. (D) Inhibition by siRNA for PPARγ (SiPγ) on FIAF target gene expression compared with scrambled (Scr.) oligonucleotides. (E) Real-time PCR of FIAF in HT-29s treated with EC16 along with kinase inhibitors PD98059, LY294002, and SB203580 for 6 h. (F) Western blot of nuclear extracts of HT-29s stimulated by EC16 together with LY294002 or SB203580 for 30 min. Ku70 is shown as a loading control. Bars signify means with standard errors and are representative of at least three independent experiments.