Table 1.
Geometrical parameters of the hook curve
| Hook parameter | symbol | typical range | characteristics |
| Start point | Σ(0) ≈ Σstart, | 1.0 – 2.5 | Non-specific signal |
| Δ(0) ≈ Δstart | 0.0 – 0.15 | PM/MM-gain (N) | |
| End point | Σ(∞), | 3.5 – 4.8 | Saturation signal |
| Δ(∞) | 0 | PM/MM-gain (as) | |
| Width | β = Σ(∞)-Σ(0) | 2.2 – 3.2 | Measuring range, non- specific binding strength in logarithmic scale |
| asymptotic height | α | 0.75 – 1.1 | PM/MM-gain (S) |
| decay constant | λ | 0.5 – 1.5 | Decay rate of the density distribution of the Σ-values; this S/N-index characterizes the mean ratio of specific and non- specific binding (S/N- ratio) in the logarithmic scale. |
| Expression index | φ = (β - Δ(0)) - λ ≈ β - λ | 1.5 – 2.5 | Mean specific signal in logarithmic scale |