TABLE 3.
Ca2+ dissociation kinetics of CaM and mutant CaM complexes with Cx32 C-terminal tail peptides
The rate constants of Ca2+ dissociation of CaM and CaM mutant complexes with Cx32 C-terminal tail peptides Cx32 208–226 and 208–227 (see “Materials and Methods” for sequence).
|
N-terminal CaM lobe
|
C-terminal CaM lobe
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
|
EF1a
|
EF2a
|
EF3a
|
EF4a
|
||||||
| k1 | A1 | k2 | A2 | k3 | A3 | k4 | A4 | n | |
| s-1 | s-1 | s-1 | s-1 | ||||||
| CaM + 208-226 | 1000 | 1 | 1000 | 1 | 6.1 ± 0.9 | 1 | 6.1 ± 0.9 | 1 | 3 |
| CaM12 + 208-226 | 7.6 ± 0.1 | 1 | 1.6 ± 0.1 | 1 | 1 | ||||
| CaM34 + 208-226 | 17.1 ± 0.9 | 1 | 173 ± 0.4 | 1 | 2 | ||||
| CaM + 208-227 | 13.5 ± 0.4 | 0.5 | 1000 | 1.5 | 2.6 ± 0.1 | 1 | 2.6 ± 0.1 | 1 | 2 |
| CaM12 + 208-227 | 3.1 ± 0.1 | 0.5 | 3.1 ± 0.1 | 1 | 1 | ||||
| CaM34 + 208-227 | 6.3 ± 0.7 | 0.5 | 169 ± 5.6 | 1 | 2 | ||||
a
Note that the denotations given to the EF hands are for reference purposes only and do not correlate to the actual hand involved in the reaction.