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. 1987 Sep;61(9):2823–2827. doi: 10.1128/jvi.61.9.2823-2827.1987

Genetic control of capsid length in bacteriophage T4: clustering of ptg mutations in gene 23.

A H Doermann, A Pao, P Jackson
PMCID: PMC255795  PMID: 3612952

Abstract

Fifty-two new bacteriophage T4 ptg mutations have been isolated by selecting for the giant-capsid phenotype they display. Genetic mapping placed all of them at eight sites, all located in gene 23. These sites were clustered in three locations, one near amber B17 (gene 23 nucleotide [NT] 268), another centrally placed between amE506 (NT 706) and amE1270 (NT 925), and the third between amC208 (NT 1297) and amE1236 (NT 1489). The lack of a selective system for identifying recombinant genotypes when dealing with the very close linkages found within these clusters opens the possibility that more than eight sites are represented in this set of mutations. Since one site was represented by only one mutation, it seems likely that further searching might uncover additional sites. It is suggested that the clustering of mutations observed here identifies regions of the gene 23 product that play a role in regulating the capsid length of T4.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bijlenga R. K., Aebi U., Kellenberger E. Properties and structure of a gene 24-controlled T4 giant phage. J Mol Biol. 1976 May 25;103(3):469–498. doi: 10.1016/0022-2836(76)90213-8. [DOI] [PubMed] [Google Scholar]
  2. Celis J. E., Smith J. D., Brenner S. Correlation between genetic and translational maps of gene 23 in bacteriophage T4. Nat New Biol. 1973 Jan 31;241(109):130–132. doi: 10.1038/newbio241130a0. [DOI] [PubMed] [Google Scholar]
  3. Chase M, Doermann A H. High Negative Interference over Short Segments of the Genetic Structure of Bacteriophage T4. Genetics. 1958 May;43(3):332–353. doi: 10.1093/genetics/43.3.332. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cummings D. J., Chapman V. A., DeLong S. S. Structural aberrations in T-even bacteriophage. IX. Effect of mixed infection on the production of giant bacteriophage. J Virol. 1977 May;22(2):489–499. doi: 10.1128/jvi.22.2.489-499.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doermann A. H., Boehner L. The identification of complex genotypes in bacteriophage T4. I. Methods. Genetics. 1970 Nov;66(3):417–428. doi: 10.1093/genetics/66.3.417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Doermann A. H., Eiserling F. A., Boehner L. Genetic control of capsid length in bacteriophage T4. I. Isolation and preliminary description of four new mutants. J Virol. 1973 Aug;12(2):374–385. doi: 10.1128/jvi.12.2.374-385.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Doermann A. H., Parma D. H. Recombination in bacteriophage T4. J Cell Physiol. 1967 Oct;70(2 Suppl):147–164. doi: 10.1002/jcp.1040700411. [DOI] [PubMed] [Google Scholar]
  8. Doherty D. H. Genetic studies on capsid-length determination in bacteriophage T4. I. Isolation and partial characterization of second-site revertants of a gene 23 mutation affecting capsid length. J Virol. 1982 Aug;43(2):641–654. doi: 10.1128/jvi.43.2.641-654.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Doherty D. H. Genetic studies on capsid-length determination in bacteriophage T4. II. Genetic evidence that specific protein-protein interactions are involved. J Virol. 1982 Aug;43(2):655–663. doi: 10.1128/jvi.43.2.655-663.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Eiserling F. A., Corso J., Feng S., Epstein R. H. Intracellular morphogenesis of bacteriophage T4. II. Head morphogenesis. Virology. 1984 Aug;137(1):95–101. doi: 10.1016/0042-6822(84)90012-6. [DOI] [PubMed] [Google Scholar]
  11. Eiserling F. A., Geiduschek E. P., Epstein R. H., Metter E. J. Capsid size and deoxyribonucleic acid length: the petite variant of bacteriophage T4. J Virol. 1970 Dec;6(6):865–876. doi: 10.1128/jvi.6.6.865-876.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KRIEG D. R. Ethyl methanesulfonate-induced reversion of bacteriophage T4rII mutants. Genetics. 1963 Apr;48:561–580. doi: 10.1093/genetics/48.4.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kozinski A. W., Doermann A. H. Repetitive DNA replication of the incomplete genomes of phage T4 petite particles. Proc Natl Acad Sci U S A. 1975 May;72(5):1734–1738. doi: 10.1073/pnas.72.5.1734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mooney D. T., Stockard J., Parker M. L., Doermann A. H. Genetic control of capsid length in bacteriophage T4: DNA sequence analysis of petite and petite/giant mutants. J Virol. 1987 Sep;61(9):2828–2834. doi: 10.1128/jvi.61.9.2828-2834.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Paietta J. V., McDonald T. L., Doermann A. H. Method for the isolation of bacteriphage T4 mutants that produce particles with giant capsids. J Virol. 1976 May;18(2):785–787. doi: 10.1128/jvi.18.2.785-787.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Parker M. L., Christensen A. C., Boosman A., Stockard J., Young E. T., Doermann A. H. Nucleotide sequence of bacteriophage T4 gene 23 and the amino acid sequence of its product. J Mol Biol. 1984 Dec 15;180(3):399–416. doi: 10.1016/0022-2836(84)90019-6. [DOI] [PubMed] [Google Scholar]
  17. Parma D. H. The structure of genomes of individual petit particles of the bacteriophage T4D mutant E920/96/41. Genetics. 1969 Oct;63(2):247–261. doi: 10.1093/genetics/63.2.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Showe M. K., Onorato L. Kinetic factors and form determination of the head of bacteriophage T4. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4165–4169. doi: 10.1073/pnas.75.9.4165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tessman I. Mutagenic treatment of double- and single-stranded DNA phages T4 ans S13 with hydroxylamine. Virology. 1968 Jun;35(2):330–333. doi: 10.1016/0042-6822(68)90275-4. [DOI] [PubMed] [Google Scholar]
  20. WATSON J. D. The properties of x-ray inactivated bacteriophage. I. Inactivation by direct effect. J Bacteriol. 1950 Dec;60(6):697–718. doi: 10.1128/jb.60.6.697-718.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yanagida M., Suzuki Y., Toda T. Molecular organization of the head of bacteriophage Teven: underlying design principles. Adv Biophys. 1984;17:97–146. doi: 10.1016/0065-227x(84)90026-1. [DOI] [PubMed] [Google Scholar]

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