Table 3.
Quantitation with cross-assignment versus quantitation without cross-assignment (Hip A over-expressed versus normal E.coli, 40−60 kDa SDS-PAGE band)
| Without cross-assignment (Xb) | With cross-assignment (Yc) | |||
|---|---|---|---|---|
| Total | Proteins from Y common with Xd | Additional proteinse | ||
| No. PCS | 486 | 982 | 754 | 228 |
| No. Proteins | 190 | 351 | 190 | 161 |
| No. Proteins2a (No. PCS ≥ 2) | 114 | 222 | 166 | 56 |
| Mean no. PCS (for proteins2) | 3.6 | 3.8 | 4.4 | 2.2 |
| Mean no. peaks (per protein2) | 21.6 | 22.7 | 27.6 | 8.3 |
| Mean CV (per protein2) | 0.18 | 0.15 | 0.13 | 0.23 |
| Mean no. peaks per PCS | 6.00 | 5.97 | 6.27 | 3.77 |
a
Proteins2 - proteins quantitated with 2 or more PCSs.
b
X- protein quantitation without using cross-assignment
c
Y- protein quantitation with using cross-assignment
d
Proteins from Y common with X — proteins that were quantitated with and without cross-assignment.
e
Additional proteins — proteins that were quantitated only with cross-assignment
No. - number