Table 1.
Mecamylamine but not hexamethonium prevents the nicotine-induced DNMT1 down-regulation in mouse FC
| Treatment | DNMT1 OD /β-actin OD |
|
|---|---|---|
| Vehicle | Nicotine | |
| Vehicle | 100 | 69 ± 5* |
| Mecamylamine | 94 ± 20 | 93 ± 10 |
| Hexamethonium | 112 ± 15 | 59 ± 10* |
Mice were treated four times per 12 h for 4 days with nicotine (22 μmol/kg s.c.), mecamylamine (6 μmol/kg s.c.), hexamethonium (19 μmol/kg s.c.), or their combination. DNMT1 protein levels (OD ratio to β -actin) are expressed as percentage of controls. Each value is the mean ± SE of five mice. Overall one-way ANOVA yielded a P = 0.004. *, P < 0.05 for Student-Newman-Keuls multiple comparison.