TABLE 1.
PRIMER SETS FOR PCR AND REAL-TIME RT-PCR
| Primer Sets | |
|---|---|
| Real-time RT-PCR | |
| Rat GAPDH | F 5′-ATGCCGCCTGGAGAAACC-3′ |
| R 5′-AGAATGGGAGTTGCTGTTGAAG-3′ | |
| Rat Thy-1 | F 5′-GCCTGACCCGAGAGAAGAAGAAG-3′ |
| R 5′-TGGTGGTGAAGTTCGCTAGAGTAAG-3′ | |
| Rat DNMT1 | F 5′-AACCTACCACGCCGACATC-3′ |
| R 5′-GCCGCCCTGTGAGTAATCC-3′ | |
| Rat DNMT3a | F 5′-CCAAGTGGACCGCTACATC-3′ |
| R 5′-ACTCCTGGATATGCTTCTGTG-3′ | |
| Rat DNMT3b | F 5′-ACAGCGGAGGATGCCAAGC-3′ |
| R 5′-TCTTCCAGGTCAGGATCAGTAGTG-3′ | |
| Rat MBD1 | F 5′-AAGGGAGAGACGCAGGACATTG-3′ |
| R 5′-AGGCAGCAGATGGAGCACAC-3′ | |
| Rat Mecp2 | F 5′-GAAGAGTGGAAAGGGACTGAAGAC-3′ |
| R 5′-GCGTGATGGTGGTGATGATGG-3′ | |
| Human Thy-1 | F 5′-CCTGACCCGTGAGACAAAGAAG-3′ |
| R 5′-GCTAGTGAAGGCGGATAAGTAGAG-3′ | |
| PCR | |
| Rat Thy-1 | F 5′-GCGAGCCTCAACCTCACTG-3′ |
| R 5′-CACCTGACTCTGCCTCCTAAG-3′ | |
| Human Thy-1 | F 5′-AGAGCCTTCGTCTGGACTG-3′ |
| R 5′-TGGTTCGGGAGCGGTATG-3′ |