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. Author manuscript; available in PMC: 2009 Apr 23.
Published in final edited form as: Nature. 2008 Sep 21;455(7216):1109–1113. doi: 10.1038/nature07336

Figure 2. MyD88 deficiency leads to local tolerance to pancreatic antigens.

Figure 2

  1. The overall reactivity of T cells from secondary lymphoid organs of MyD88-sufficient (solid bars) and MyD88-negative (open bars) NOD mice was calculated as a mean of the percentages of mice in the given group reacting to individual diabetes-associated peptides (see Supplemental Fig. 2c).
  2. The frequency of CD8+ T cells producing IFN-γ in response to peptide recognized by diabetogenic clone 8.3 was determined in the spleens, MLN and PLN of MyD88-sufficient (solid bars) and MyD88-negative (open bars) NOD mice. Error bars – s.e.m.
  3. Proliferation of CFSE-labeled BDC2.5 CD4+ T cells, depleted of CD25+ T cells, in the PLNs of SPF NOD and NOD.MyD88KO mice assayed on day 3 after i.v. injection. Representative CFSE dilution profiles are shown for BDC2.5+-gated cells in PLN (red) and control skin-draining lymph nodes (blue).

p values in all experiments were determined using unpaired Student's t test. Error bars – s.e.m. n, number of animals per group.