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. 2008 Nov 11;3(11):e3694. doi: 10.1371/journal.pone.0003694

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Hunter et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Peripheral blood microvesicles from healthy donors (n = 10) were analyzed by flow cytometry. (A) The size of the microvesicles was determined by FSC vs. SSC. The gate was set according to 2 micron beads (inset). (B) To determine cell origin, microvesicles were stained for CD3, CD202b (Tie-2), CD66b, CD79a, or CD41a to determine those that originated from T-cells, endothelial cells, neutrophils, B-cells, or platelets. Mononuclear phagocyte-derived microvesicles were positive for CD14, CD206, CCR3, CCR2, or CCR5. Shown is the average % maximum of total gated events±S.E.M.