Table 1.
IL-1β mRNA expression in nearly pure β-cells obtained from patients with and without T2DM
| Gene array IL-1ß | PCR IL-1ß | Gene array IL-8 | PCR IL-8 | |
|---|---|---|---|---|
| Diabetic | ||||
| D2 | 154 | 241.35 | 76.8 | 17.2 |
| D3 | 107 | 51.55 | 148.7 | 35.14 |
| D1 | 29 | 44.83 | 31.4 | nd |
| DM2 | 78 | 117.47 | 27.5 | 0.65 |
| D6 | 28 | 3.65 | 16.6 | nd |
| D7 | 77 | 6.27 | 70.6 | 12.08 |
| D8 | 16 | nd | 34.4 | 22.5 |
| D9 | 47 | nd | 57.3 | nd |
| D11 | 30 | nd | 32.9 | 3.14 |
| D12 | 20 | nd | 11 | nd |
| Mean ± se | 58 ± 14.2 | 77.52 ± 36.84 | 50.7 ± 12.9 | 15.12 ± 5.23 |
| Nondiabetic | ||||
| H13 | 13 | 1.00 | 12.89 | nd |
| H16–2 | 30 | nd | 28.3 | 1.00 |
| H03 | 35 | 1.27 | 48.1 | nd |
| H18 | 23 | nd | 17.5 | 1.96 |
| H6 | 15 | nd | 25.4 | 0.87 |
| H7 | 26 | nd | 10.7 | nd |
| H8 | 39 | nd | 15.4 | nd |
| H14 | 50 | 1.88 | 35.5 | 8.93 |
| H16 | 21 | nd | 13.3 | nd |
| Mean ± se | 28.0 ± 3.96 | 1.39 ± 0.26 | 22.24 ± 3.81 | 3.19 ± 1.9 |
| P = 0.032 | P = 0.024 | P = 0.0337 | P = 0.086 |
IL-1β and IL-8 mRNA expression in laser-captured β-cell was analyzed by gene array and real-time PCR. Real-time PCR results were expressed as fold of a positive control sample arbitrarily defined as 1. Results passed the normality test. P, Controls vs. diabetics (t test); nd, not detectable.