TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype or phenotype | Reference or source |
|---|---|---|
| E. coli DH5α | λ− φ80dlacZΔM15 Δ(lacZYA-argF)U196 recA1 endA1 hsdR17 (rK− mK−) supE44 thi-1 gyrA relA1 | 57 |
| P. aeruginosa strains | ||
| PAO1 | Wild type | 24 |
| PJF-QE1 | pqsE deletion mutant derived from strain PAO1 | This study |
| PJF-QA1 | pqsA deletion mutant derived from strain PAO1 | This study |
| PJF-QR1 | pqsR deletion mutant derived from strain PAO1 | This study |
| PAO-R1 | lasR::Tet; derived from strain PAO1 | 21 |
| PAO-JP1 | lasI::Tet; derived from strain PAO1 | 42 |
| PDO111 | rhlR::Tn501-11; derived from strain PAO1 | 2 |
| PDO100 | rhlIΔ::Tn501-2; derived from strain PAO1 | 2 |
| PAO-JP2 | lasI::Tet rhlIΔ::Tn501-2; derived from strain PDO100 | 42 |
| PAO-JP3 | lasR::Tet rhlR::Tn501-11; derived from strain PDO111 | 42 |
| PAO-JP2E | lasI::Tet rhlIΔ::Tn501-2 ΔpqsE; derived from strain PAO-JP2 | This study |
| Plasmids | ||
| pMTP58 | Cosmid vector containing pqs genes | 25 |
| pEX18Ap | Suicide vector for P. aeruginosa | 23 |
| pΔpqsE-suc | pqsE deletion suicide vector | This study |
| pΔpqsA-suc | pqsA deletion suicide vector | This study |
| pΔpqsR-suc | pqsR deletion suicide vector | This study |
| pACYC184 | Cloning vector for E. coli | 6 |
| pEX1.8 | P. aeruginosa expression vector | 46 |
| pZOE01 | tacp-pqsE on pEX1.8 | This study |
| pROSE04 | tacp-pqsE on pACYC184 | This study |
| pDSW7 | tacp-pqsA on pEX1.8 | This study |
| pDSW8 | tacp-pqsR on pEX1.8 | 54 |
| pECP60 | rhlR′-lacZ translational fusion | 56 |
| pECP61.5 | rhlR′-lacZ translational fusion; tacp-rhlR | 42 |
| pECP62.5 | lasB′-lacZ tacp-rhlR | 42 |
| pECP64 | lasB′-lacZ tacp-lasR | 42 |
| pPCS1002 | rhlR′-lacZ transcriptional fusion | 45 |