TABLE 2.
Pyocyanin production
| Strain | Pyocyanin production (μM) in strain carryinga:
|
|
|---|---|---|
| pEX1.8 | pZOE01 | |
| PAO1 | 42.6 ± 8.3 | 284.4 ± 19.3 |
| PJF-QE1 | ND | 276.5 ± 18.2 |
| PJF-QA1 | ND | 261.5 ± 29.7 |
| PJF-QR1 | ND | 233.6 ± 44.3 |
| PDO111 | ND | ND |
| PDO100 | ND | 15.3 ± 6.5 |
a
Data are the means ± SDs of results from at least three independent experiments. pEX1.8, control plasmid; pZOE01, PqsE overexpression plasmid. ND, not detectable. Strains PJF-QA1 and PJF-QR1 produced 34.3 ± 8.4 and 60.8 ± 5.6 μM pyocyanin, respectively, when complemented by plasmids containing a wild-type copy of the gene that had been mutated.