Figure 1.
Resolvin E1 (RvE1) decreased osteoclast (OC) growth and dentin resorption in vitro. (a) RvE1 decreased OC growth in bone marrow cultures treated with murine colony-stimulating factor (M-CSF) and receptor activator of nuclear factor-κB ligand (RANKL). RvE1 was administered throughout the 7-day culture period in doses indicated. The RvE1 precursor 18R-hydroxy-eicosapentaenoic acid (18-HEPE) was administered in a similar manner at 3 ng mL−1. The cumulative surface area of tartrate-resistant acid phosphatase (TRAP)-positive cells with >3 nuclei are compared with that of control cells. (b) Eicosapentaenoic acid (EPA) at similar doses increased OC covered area when administered for 7 days. (c) RvE1 decreases dentin disc resorption. Bone marrow cultures were seeded on dentine discs and OC differentiation was induced with M-CSF and RANKL for 14 days in the presence or absence of RvE1 (3 ng mL−1). Resorption pits were counted on scanning electron microscopy (SEM) images of discs. In (a–c), means and s.e.mean are shown, n=4 (a, b), n=5 (c); *P<0.05. (d) SEM images of resorption pits of dentin discs from control and RvE1-treated cultures (scale bar: 100 μm).